ENTROPIC ELASTICITY OF LAMBDA PHAGE DNA PDF
Semantic Scholar extracted view of “Entropic elasticity of lambda-phage DNA.” by C. Bustamante et al. Entropic elasticity of λ-phage DNA. C. Bustamante, J. F. Fingerprint. Bacteriophage lambda. Viral DNA. Elasticity. Least-Squares Analysis. scientific article (publication date: 9 September ).
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Moving the native bacterial integration site to different locations on the genome and measuring the integration frequency in these strains reveals that the frequencies of the native site llambda a site symmetric to it relative to the origin are similar, whereas both are significantly higher than when the integration site is moved near the elasticiity, consistent with the replication-driven mechanism we propose. PCR amplification of up to kb DNA with high fidelity and high yield from lambda bacteriophage templates.
Background Consistent progress in the development of bacteriophage lambda display platform as an alternative to phag phage display system was achieved in the recent years. For Permissions, please e-mail: From NMR and limited proteolysis we show that about 15 C-terminal residues of CII are highly flexible, and may act as a target for proteolysis in vivo. These results enhance our understanding of phage -mediated transduction as a mechanism of HGT in the Lyme disease spirochetes.
Purification and DNA -binding properties of the cro-type regulatory repressor protein cng encoded by the Lactobacillus plantarum phage phi g1e. In the present work we describe an original method for dual display of large proteins on the surface of lambda particles. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.
We have performed a large scale phylogenetic analysis and pairwise sequence comparisons of SSB proteins from different phyla. A crucial element in the lysis-lysogeny decision of the temperate coliphage lambda is the phage protein CII, which has pbage interesting properties.
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Heavy ions also showed exponential inactivation kinetics to a survival of 10 After exposure of different doses of simulated Martian UV radiation a decrease of the biological activity of phages and characteristic changes in the UV entropuc spectrum have been detected, indicating the UV damage of isolated and intraphage T7 DNA. The development of molecular cloning techniques, ironically instigated largely by phage lambda researchers, allowed many phage workers to switch their efforts to other biological systems.
Verified email at alice. We demonstrate that mosaics are mainly generated by homology-driven mechanisms that tolerate high substrate divergence. By varying packaged DNA elastiity and through addition of spermine ions, we transform the interaction energy from net repulsive to net attractive.
We have identified the loci on the DNA which are in contact with the capsid by chemical crosslinking, induced by ultraviolet-irradiation of phage containing 5-bromodeoxyuridine in place of thymine. The abnormal decrease in the DNA coil size in low ionic strength buffers is not a specific feature of lambda phage DNA only.
The observed distribution of these specific temperate Vibriophages across large geographical scales may be explained by efficient dispersal of phages and bacteria in the marine environment combined with a mutualistic interaction between temperate phages and elasicity hosts which selects for co-existence rather than arms race dynamics. Phage LYGO9 was shown having a short latent period and a larger burst size.
Changes in the rigidity of the polymetric chain of phage lambda double-strand DNA have been studied by laser correlation spectroscopy.
Its double-stranded DNA genome is 26, bp long and has 33 open reading frames. Helicobacter pylori is a common human pathogen that has been identified to be carcinogenic.
We stress the importance of eliminating salt residues in these measurements. Acinetobacter phage genome is similar to Sphinx 2. The implications of these results with respect to the two roles of the terminase holoenzyme — DNA maturation and DNA packaging — are discussed.
Contacts are found nearly randomly over the entire map. This strategy enables any DNA fragment containing the phage attL recombination sites to be efficiently inserted into the attR sites of the allele replacement vector using phage lambda clonase. Functional studies demonstrated that, despite the structural and amino acid sequence differences from bacterial SSBs, Orf14bIL67 protein complements the conditional lethal ssb-1 mutation of Escherichia coli.
P-lethality is assessed by examining host cells for transformation by ColE1-type plasmids that can express P, and the absence of transformants is attributed to a lethal effect of P expression. Southern blot analysis of chromosomal DNA of a lysogenic N. For both phages studied the dose-response curve of mutagenesis was non-linear.
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The fusion product – a phasmid – can behave as a plasmid, as a phage and as a prophage. Recombinant Cernunnos protein restored gap filling and end joining of partially complementary overhangs, and stimulated joining of cohesive ends more than twentyfold.
XLF-dependent gap filling was nearly eliminated by immunodepletion of DNA polymerase lambdabut was restored by addition of either polymerase lambda or polymerase mu. The technology’s success depends on the efficiency with which polypeptides can be displayed on the phage surface, and significant progress has been made in engineering M13 bacteriophage coat proteins as improved phage display platforms.
This mechanism may prove to be more general, as other mobile genetic elements such as ICE encode Radlike. These findings, and the fact that the VL clones eelasticity randomly selected from normal lymphoid tissues, strongly indicate that the bulk of chicken L-chains is encoded by a few germline VL genes, probably much less than 15 since many of the VL genes are known to be pseudogenes.
It seems unlikely that the rare codons present in the genes, and any feature of their mRNA secondary structure play a role in page control of their translation. We describe a simple method of using rolling circle amplification to amplify vector DNA such as M13 or plasmid DNA from single colonies or plaques.